Promotor: G.A. van der Marel, Co-promotor: D.C. Codée

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

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This thesis is about augmented reality (AR). AR is commonly considered a technology that integrates virtual images into a user’s view of the real world.

The research described in this thesis focused on the use of bioorthogonal antigens to investigate immunological processes in antigen presenting cells. Bioorthogonal antigens are antigenic proteins produced through recombinant expression in a methionine auxotrophic E. coli strain.

Promotor: G.A. van der Marel, Co-promotor: J.D.C. Codée

PURPOSE: To monitor the biodistribution of IgG1 aggregates upon subcutaneous (SC) and intravenous (IV) administration in mice and measure their propensity to stimulate an early immune response.

On this section of the website you can find more information about the Old Observatory and the current astronomy research institute.

The Graduate School of Science offers 9 PhD programmes, spanning the entire spectrum of science. Do you have a Master's degree and are you proficient in English? Do you share a fascination for science? Then you may find the Graduate School of Science an inspiring and ideal environment for obtaining…

The work described in this dissertation contributes to a better mechanistic understanding of nanoparticles in vivo. To achieve that goal, we used the zebrafish as a highly predictive pre-screening model of nanoparticles.

Natural history collections provide invaluable sources for researchers with different disciplinary backgrounds, aspiring to study the geographical distribution of flora and fauna across the globe as well as other evolutionary processes.

This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…

We have studied shape with a particular focus on the zebrafish model system. The shape is an essential appearance of the phenotype of a biological specimen and it can be used to read out a current state or response or to study gene expression.

This work has described synthetic strategies towards well-defined structures resembling capsular polysaccharide (CPS) fragments, CPS mimics, teichoic acid (TA) fragments as well as a third-generation ring-closing tandem metathesis (RCM) linker to better exploit the potential of automated synthesis.

One of the main challenges in the development of an effective anti-cancer vaccine is the generation of an adequate and directed cellular immune response.

This thesis describes the use of a combined approach of computational and experimental techniques to gain novel insights to understand the glycosylation reaction and its reactive intermediates.

Carbohydrates, or sugars, are the most diverse and most abundant biomolecules known. However, the isolation of carbohydrate samples in sufficient amounts and purity is often impractical or even impossible, so the chemical synthesis of glycosides becomes relevant. The glycosylation reaction, in which…

Glycosidases (GHs) are enzymes responsible for the degradation of carbohydrates and play many roles in human health and pathophysiology. Often, abnormal levels of glycosidase activity are markedly linked to human pathologies.

Schistosomiasis is an acute and chronic disease caused by blood dwelling parasitic trematodes of the genus Schistosoma, and it is classified as the second most socioeconomically devastating parasitic disease, second only to malaria.

The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…

This Thesis focuses on the design and synthesis of ADP-ribosylated compounds that can be applied in biological studies.

Lysosomal glycosidases are acid hydrolases that fragment glycoconjugates in lysosomes.

This Thesis describes a strategy to synthesize 1,2-cis-oligosaccharides modulated by additives, such as dimethylformamide (DMF), triphenylphosphine oxide (Ph3P=O) and N-methyl(phenyl)formamide (MPF), using building blocks that carry solely benzyl type protecting groups and are therefore of uniform r…

The function of TLRs in innate immunity has aroused worldwide attention soon after its discovery. Because of the broad functions of TLR2 in innate immunity, the drive for the development of TLR2-targeted vaccines or therapeutic treatments has accelerated in the last decades.

Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site…

Optical projection tomography (OPT) is a tomographic 3D imaging technique used for specimens in the millimetre scale.

This thesis introduces a relational interaction model and a practical tool for describing, visualising and generating interactive dialogues.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Antibiotic resistance, caused by widespread use of antibiotics, leads to bacterial infections that are difficult, if not impossible, to treat and is a major worldwide health concern.

Het is een wijdverbreid idee dat (post-)middeleeuwse steden een sterfteoverschot hadden en zij slechts in leven konden blijven door de toestroom van migranten; een fenomeen dat bekend staat als het ‘urban graveyard’-effect. Over details valt te twisten, maar duidelijk is dat de stad en de dood dichter…

In this thesis I highlight the applications of Oxford Nanopore Technologies (ONT) sequencing. This technique is a relatively new approach in the sequencing field, where nanopores are embedded in a membrane, DNA molecules are pulled through nanopores and an electrical current serving as the sequencing…

The Faculty of Humanities consist of various academic fields. What is the common ground of all these different academics and students? They tell their story and why it matters in today’s world in 'We are Humanities'.

Promotor: B. van de Water, Co-Promotores: J.H.N. Meerman, F.J. Verbeek

In this thesis we will explore the use of fuzzy systems theory for applications in bioinformatics.

Leiden University wants its research and teaching to have a positive impact on society. In an effort to achieve this, many of our researchers collaborate with academic and community partners, non-governmental organisations and the business sector.

Detection of cerebral beta-amyloid (Abeta) by targeted contrast agents remains of great interest to aid the in vivo diagnosis of Alzheimer's disease (AD). Bis-styrylbenzenes have been previously reported as potential Abeta imaging agents.

The new building for the Faculty of Mathematics and Natural Sciences is a significant milestone for Leiden University and the faculty in particular. A new building is being realized in three phases. The first phase of the new building was put into use in 2016 and covers an area of 45,000m2. The second…

The marine shipping industry is one of the strongest emitters of nitrogen oxides (NOx), a pollutant detrimental to ecology and human health. Over the last 20 years, the pollution produced by power plants, the industry sector, and cars has been decreasing.

Promotor: J.N. Kok, Co-promotor: F.J. Verbeek

The specialisation High-Tech Innovation is for MSc students in Sciences who are interested in interdisciplinary technologies and research related to /important for high technology industries, e.g. the semi-conductor industry or nano-fabrication. In addition to their primary field of study, students…

‘Regenerative medicine’ explores ways to repair or replace damaged cells, tissues and organs with cell, stem-cell or gene therapy. It can lead to valuable breakthroughs in the treatment of chronic diseases such as diabetes, heart problems and kidney diseases or muscle diseases.

Humans perceive the real world through their sensory organs: vision, taste, hearing, smell, and touch. In terms of information, we consider these different modesalso referred to as different channels of information or modals.

Leidse milieuwetenschappers doen onderzoek naar microplastics, bestrijdingsmiddelen en bemesting. En binnenkort naar lichtvervuiling en nieuwe materialen uit de nanotechnologie.

A new research line is the development of liquid biopsy fingerprints to predict early Alzheimer’s disease (AD) stage in human in readily accessible body fluids in human (in collaboration with: Dr. Geert-Jan Groeneveld, CHDR; Prof. Elga de Vries, Free University Medical Center; and others).

The Computer Systems, Imagery & media (CSI) research programme performs research on methods and techniques for the design, implementation and application of advanced computer systems, in particular parallel, distributed and embedded computer systems.

This dissertation presents the synthesis and evaluation of antibody recognition for various capsular polysaccharide (CP) fragments of Staphylococcus aureus (S. aureus).

A key step in the synthesis of oligosaccharides and glycoconjugates is the formation of the glycosidic bond.

This dissertation explores new chemical strategies for the synthesis of self-adjuvanting peptide conjugates and immunomodulatory ligands aimed at targeted activation of the immune system.

Neurodegenerative diseases pose a large medical and societal challenge. The etiology of these diseases is still poorly understood, which makes drug discovery for these diseases difficult.